af700 conjugated cd31 Search Results


foxp3/scurfin (af-647  (Becton Dickinson)
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cd103 (af-647  (Becton Dickinson)
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Cd103 (Af 647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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integrin β7 (apc  (Becton Dickinson)
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Phenotypic analysis of CD4+ T cells and subsets from PD patients compared to caregivers in Cohort B
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facscalibur flow cytometer  (Becton Dickinson)
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Becton Dickinson facscalibur flow cytometer
Phenotypic analysis of CD4+ T cells and subsets from PD patients compared to caregivers in Cohort B
Facscalibur Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorochrome-conjugated monoclonal antibodies  (Becton Dickinson)
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Becton Dickinson fluorochrome-conjugated monoclonal antibodies
Phenotypic analysis of CD4+ T cells and subsets from PD patients compared to caregivers in Cohort B
Fluorochrome Conjugated Monoclonal Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3e (t-cell marker)(ucht1)  (Biotium)
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Biotium cd3e (t-cell marker)(ucht1)
Phenotypic analysis of CD4+ T cells and subsets from PD patients compared to caregivers in Cohort B
Cd3e (T Cell Marker)(Ucht1), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tcrb-af700  (Becton Dickinson)
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Becton Dickinson tcrb-af700
Phenotypic analysis of CD4+ T cells and subsets from PD patients compared to caregivers in Cohort B
Tcrb Af700, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd31-bv605  (Becton Dickinson)
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Becton Dickinson cd31-bv605
Assessment of in vivo CRISPR-Cas9 editing. a) experimental scheme: Ai9 mice were injected with U155@lipids encapsulating Cas9 mRNA/sgRNA (1/1 wt/wt ratio), and 9 days after injection lungs were collected and analyzed with next-generation sequencing (NGS) and IHC; b) representative images of paraffin-embedded lung sections, which were stained with antibodies for multiplex IHC. White arrows point out the tdTomato and <t>CD31+</t> co-localization. Scale bar is 200 μm; c) quantification of IHC in b . Data are presented as Means ± SD (n=3-4 biological replicates). Representative image of how QuPath’s tool select tdTomato+ stained areas is in Supplementary Figure S12; d) quantification of editing events in lungs by NGS. ata are presented as Means ± SD (n=4-5 biological replicates); e ) schematic of the Cas9 target site within the mouse PDCD-1 gene at the exon2 location; f) schematic of interaction of PD-1 positive and ΔPD-1 knockout T cells with tumor cells; g) quantific tion of editing events in CD4+/CD8+ T cells by NGS. Data are presented as Means ± SD (n=4-5 biological replicates).
Cd31 Bv605, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-cy7-conjugated anti-cd31  (Becton Dickinson)
90
Becton Dickinson pe-cy7-conjugated anti-cd31
Assessment of in vivo CRISPR-Cas9 editing. a) experimental scheme: Ai9 mice were injected with U155@lipids encapsulating Cas9 mRNA/sgRNA (1/1 wt/wt ratio), and 9 days after injection lungs were collected and analyzed with next-generation sequencing (NGS) and IHC; b) representative images of paraffin-embedded lung sections, which were stained with antibodies for multiplex IHC. White arrows point out the tdTomato and <t>CD31+</t> co-localization. Scale bar is 200 μm; c) quantification of IHC in b . Data are presented as Means ± SD (n=3-4 biological replicates). Representative image of how QuPath’s tool select tdTomato+ stained areas is in Supplementary Figure S12; d) quantification of editing events in lungs by NGS. ata are presented as Means ± SD (n=4-5 biological replicates); e ) schematic of the Cas9 target site within the mouse PDCD-1 gene at the exon2 location; f) schematic of interaction of PD-1 positive and ΔPD-1 knockout T cells with tumor cells; g) quantific tion of editing events in CD4+/CD8+ T cells by NGS. Data are presented as Means ± SD (n=4-5 biological replicates).
Pe Cy7 Conjugated Anti Cd31, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b220-buv805  (Becton Dickinson)
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Becton Dickinson b220-buv805
Assessment of in vivo CRISPR-Cas9 editing. a) experimental scheme: Ai9 mice were injected with U155@lipids encapsulating Cas9 mRNA/sgRNA (1/1 wt/wt ratio), and 9 days after injection lungs were collected and analyzed with next-generation sequencing (NGS) and IHC; b) representative images of paraffin-embedded lung sections, which were stained with antibodies for multiplex IHC. White arrows point out the tdTomato and <t>CD31+</t> co-localization. Scale bar is 200 μm; c) quantification of IHC in b . Data are presented as Means ± SD (n=3-4 biological replicates). Representative image of how QuPath’s tool select tdTomato+ stained areas is in Supplementary Figure S12; d) quantification of editing events in lungs by NGS. ata are presented as Means ± SD (n=4-5 biological replicates); e ) schematic of the Cas9 target site within the mouse PDCD-1 gene at the exon2 location; f) schematic of interaction of PD-1 positive and ΔPD-1 knockout T cells with tumor cells; g) quantific tion of editing events in CD4+/CD8+ T cells by NGS. Data are presented as Means ± SD (n=4-5 biological replicates).
B220 Buv805, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc-linage cocktail (cd3, cd16, cd19, cd20, cd14 and cd56) antibody  (Becton Dickinson)
90
Becton Dickinson fitc-linage cocktail (cd3, cd16, cd19, cd20, cd14 and cd56) antibody
Assessment of in vivo CRISPR-Cas9 editing. a) experimental scheme: Ai9 mice were injected with U155@lipids encapsulating Cas9 mRNA/sgRNA (1/1 wt/wt ratio), and 9 days after injection lungs were collected and analyzed with next-generation sequencing (NGS) and IHC; b) representative images of paraffin-embedded lung sections, which were stained with antibodies for multiplex IHC. White arrows point out the tdTomato and <t>CD31+</t> co-localization. Scale bar is 200 μm; c) quantification of IHC in b . Data are presented as Means ± SD (n=3-4 biological replicates). Representative image of how QuPath’s tool select tdTomato+ stained areas is in Supplementary Figure S12; d) quantification of editing events in lungs by NGS. ata are presented as Means ± SD (n=4-5 biological replicates); e ) schematic of the Cas9 target site within the mouse PDCD-1 gene at the exon2 location; f) schematic of interaction of PD-1 positive and ΔPD-1 knockout T cells with tumor cells; g) quantific tion of editing events in CD4+/CD8+ T cells by NGS. Data are presented as Means ± SD (n=4-5 biological replicates).
Fitc Linage Cocktail (Cd3, Cd16, Cd19, Cd20, Cd14 And Cd56) Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorochrome-conjugated antibodies fitc-linage cocktail  (Becton Dickinson)
90
Becton Dickinson fluorochrome-conjugated antibodies fitc-linage cocktail
Assessment of in vivo CRISPR-Cas9 editing. a) experimental scheme: Ai9 mice were injected with U155@lipids encapsulating Cas9 mRNA/sgRNA (1/1 wt/wt ratio), and 9 days after injection lungs were collected and analyzed with next-generation sequencing (NGS) and IHC; b) representative images of paraffin-embedded lung sections, which were stained with antibodies for multiplex IHC. White arrows point out the tdTomato and <t>CD31+</t> co-localization. Scale bar is 200 μm; c) quantification of IHC in b . Data are presented as Means ± SD (n=3-4 biological replicates). Representative image of how QuPath’s tool select tdTomato+ stained areas is in Supplementary Figure S12; d) quantification of editing events in lungs by NGS. ata are presented as Means ± SD (n=4-5 biological replicates); e ) schematic of the Cas9 target site within the mouse PDCD-1 gene at the exon2 location; f) schematic of interaction of PD-1 positive and ΔPD-1 knockout T cells with tumor cells; g) quantific tion of editing events in CD4+/CD8+ T cells by NGS. Data are presented as Means ± SD (n=4-5 biological replicates).
Fluorochrome Conjugated Antibodies Fitc Linage Cocktail, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Phenotypic analysis of CD4+ T cells and subsets from PD patients compared to caregivers in Cohort B

Journal: Journal of Neuroimmune Pharmacology

Article Title: CD4+ Regulatory and Effector/Memory T Cell Subsets Profile Motor Dysfunction in Parkinson’s Disease

doi: 10.1007/s11481-012-9402-z

Figure Lengend Snippet: Phenotypic analysis of CD4+ T cells and subsets from PD patients compared to caregivers in Cohort B

Article Snippet: We performed multicolor flow cytometric analysis using a FACSCalibur flow cytometer (Becton Dickinson) with fluorochrome-conjugated monoclonal antibodies against the following antigens: CD4 (FITC or Alexa Fluor [AF]-700), CD25 (PE), CD127 (PerCP-Cy 5.5), FoxP3/Scurfin (AF-647), CD95/FAS/Apo1 (APC), CD31/PECAM-1 (AF647), CD39/ENTPD1 (APC), CD49d/Integrin α4 (APC or PE-Cy 7), CD103 (AF-647), CD45RO (APC), CD45RA (AF-700), Integrin β7 (APC), CD29/integrin β1 (AF700) (BD Biosciences, San Jose, CA, USA).

Techniques:

Assessment of in vivo CRISPR-Cas9 editing. a) experimental scheme: Ai9 mice were injected with U155@lipids encapsulating Cas9 mRNA/sgRNA (1/1 wt/wt ratio), and 9 days after injection lungs were collected and analyzed with next-generation sequencing (NGS) and IHC; b) representative images of paraffin-embedded lung sections, which were stained with antibodies for multiplex IHC. White arrows point out the tdTomato and CD31+ co-localization. Scale bar is 200 μm; c) quantification of IHC in b . Data are presented as Means ± SD (n=3-4 biological replicates). Representative image of how QuPath’s tool select tdTomato+ stained areas is in Supplementary Figure S12; d) quantification of editing events in lungs by NGS. ata are presented as Means ± SD (n=4-5 biological replicates); e ) schematic of the Cas9 target site within the mouse PDCD-1 gene at the exon2 location; f) schematic of interaction of PD-1 positive and ΔPD-1 knockout T cells with tumor cells; g) quantific tion of editing events in CD4+/CD8+ T cells by NGS. Data are presented as Means ± SD (n=4-5 biological replicates).

Journal: bioRxiv

Article Title: Synthesis of ionizable lipopolymers using split-Ugi reaction for pulmonary delivery of various size RNAs and gene editing

doi: 10.1101/2024.06.11.598497

Figure Lengend Snippet: Assessment of in vivo CRISPR-Cas9 editing. a) experimental scheme: Ai9 mice were injected with U155@lipids encapsulating Cas9 mRNA/sgRNA (1/1 wt/wt ratio), and 9 days after injection lungs were collected and analyzed with next-generation sequencing (NGS) and IHC; b) representative images of paraffin-embedded lung sections, which were stained with antibodies for multiplex IHC. White arrows point out the tdTomato and CD31+ co-localization. Scale bar is 200 μm; c) quantification of IHC in b . Data are presented as Means ± SD (n=3-4 biological replicates). Representative image of how QuPath’s tool select tdTomato+ stained areas is in Supplementary Figure S12; d) quantification of editing events in lungs by NGS. ata are presented as Means ± SD (n=4-5 biological replicates); e ) schematic of the Cas9 target site within the mouse PDCD-1 gene at the exon2 location; f) schematic of interaction of PD-1 positive and ΔPD-1 knockout T cells with tumor cells; g) quantific tion of editing events in CD4+/CD8+ T cells by NGS. Data are presented as Means ± SD (n=4-5 biological replicates).

Article Snippet: Cells were further stained with the following fluorochrome-conjugated antibodies: CD45-BUV395 (BD Horizon, #565967), B220-BUV805 (BD Horizon, #569199), CD31-BV605 (BD Horizon, #740356), TcrB-AF700 (BD Horizon, #560705).

Techniques: In Vivo, CRISPR, Injection, Next-Generation Sequencing, Staining, Multiplex Assay, Knock-Out